Method for Analysis of Excitation-Emission Spectral Scans to Characterize Fluorescent Probes in Confocal Microscopy

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Popis

The main aim of the study was to use the spectral excitation-emission 2D scanning followed by 2D spectral
analysis in order to obtain an appropriate representation of the properties of the used dye in the original
image of a stained cell. We used Di-4-ANNEPS voltage sensitive dye (Invitrogen) binding to an adult rat
cardiomyocyte membrane to determine the stability of the dye and to characterize spatial differences of
its spectral properties.

Klíčová slova
confocal microscopy
fluorescence
spectral scan
Stokes shift